Size exclusion chromatography as a tool for evaluation of fragmentation pattern of gastric mucins under non-degrading conditions.
نویسندگان
چکیده
Gastric mucins are high molecular weight extracellular glycoproteins that play a major role in the protection of the gastrointestinal tract and besides that they are also involved in many disease processes. In the present study, size exclusion chromatography under non-degrading conditions was used to study the fragmentation pattern of native gastric mucins. The samples of gastric mucins of different origin obtained by an extraction of gastric mucosa with Tris-HCl buffer, pH 7.3 were separated using size exclusion chromatography on Sephadex G-100. While samples of rat gastric mucins are characterized by the presence of only high-molecular weight fraction of glycoproteins, fragmented mucin components in non-denaturated samples were observed in canine and human gastric mucins. Differences in the fragmentation pattern were observed in patients with ulcer diseases and gastric cancer. Degradation products of mucins were also detected using polyacrylamide gel electrophoresis in the presence of SDS.
منابع مشابه
Organic matter from biofilter nitrification by high performance size exclusion chromatography and fluorescence excitation-emission matrix
A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without or...
متن کاملSEPARATION OF NONHISTONE HIGH MOBILITY GROUP (HMG) FROM HUMAN LYMPHOCYTES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...
متن کاملEvaluation of Leaf Protein Pattern in Wheat Genotypes Under Drought Stress
Drought is one of the most important factors limiting crop yields around the world. Drought stress in plants, the change (increase or decrease) in the production of plant proteins. This research was carried out using bread wheat genotypes. For evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions....
متن کاملEvaluation of Leaf Protein Pattern in Wheat Genotypes Under Drought Stress
Drought is one of the most important factors limiting crop yields around the world. Drought stress in plants, the change (increase or decrease) in the production of plant proteins. This research was carried out using bread wheat genotypes. For evaluation of leaf protein pattern in wheat, 10 genotypes were assayed with 3 replications under irrigated (non-stress) and rain-fed (stress) conditions....
متن کاملIdentification of Factors Associated with Biofilm Formation Ability in the Clinical Isolates of Helicobacter pylori
Background: A few reports confirm the ability of Helicobacter pylori to form biofilm. However, conclusive data do not exist concerning the factors that favor this ability.Objectives: Evaluation of the factors associated with the biofilm formation ability of H. pylori including bacterial, physical and chemical, and environmental factors was the research’s aim.Materials and Methods: H...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Prague medical report
دوره 111 3 شماره
صفحات -
تاریخ انتشار 2010